Identification and nitrogen migration of an ammonia-oxidizing and deodorizing Bacillus

Abstract:

[Objective] to get a deodorizing bacillus of high efficient ammonia degradation, and to investigate the process of the nitrogen migration of the strain. [Methods] An ammonia-oxidizing strain was screened in a self-designed screening platform. Colonial morphology, physio-biochemical tests and 16S rRNA gene sequence analysis were adopted to determine the phylogenetic position of the isolated strain. The nitrogen migration characteristics and process of the strain that cultured by NH4Cl as the sole nitrogen source were investigated by using the chemical detection of NH4+-N, NO2?-N, NO3?-N and gases under aerobic and anaerobic conditions. [Results] A high efficient ammonia-oxidizing and deodorizing strain that has the characteristics of heterotrophic nitrification and aerobic denitrification was screened and identified as Bacillus coagulans. Under aerobic and anaerobic conditions, the NH4+-N oxidizing rate of the strain RJCC-03 could reach 98% and 23.7%, respectively. A small amount of N2, not nitrogen oxides gases, was generated during the experiment process, under aerobic conditions. [Conclusion] These results proved Bacillus coagulans had great potential application in the agricultural and environmental protection.