Influencing factors analysis and effect evaluation of Lactobacillus strains to bind plasticizers

Abstract:

[Objective] This study was designed to investigate the binding of two Lactobacillus strains, i.e., L. acidophilus NCFM and L. paralimentarius 412, to plasticizers. Several factors which affect the ability of the bacteria to bind plasticizers were also detected. [Methods] HPLC was used to detect the binding efficiency of two lactobacillus strains to three phthalates plasticizers, namely DEP, DBP and DEHP, respectively. The adsorption conditions of two strains to DBP were optimized when the bacterial cells were subjected to heat, acid or NaCl treatment. [Results] Two Lactobacillus strains showed an adsorption to three phthalic acid esters plasticizers, but strain NCFM was better in binding plasticizers than strain 412, especially in adsorbing the plasticizer DBP. The binding percentages of strain NCFM to three phthalates DEP, DBP and DEHP was 21.48%, 43.32% and 9.62%, respectively. Strain NCFM bound the maximal DBP after incubation for 4 h at 37 °C. The efficiency of strain NCFM plasticizers-adsorbing was significantly improved via heat, acid and NaCl treatment. [Conclusion] L. acidophilus NCFM showed good ability to bind phthalic acid esters plasticizers. Strain NCFM should be a candidate as one of biological tools to remove plasticizers from the environments linked to food products.