Establishment and application of an efficient cross breeding method assisted by molecular markers of Volvariella volvacea

Abstract:

[Objective] In order to establish an efficient cross breeding method assisted by molecular markers of Volvariella volvacea and apply it to monospore cross breeding for the cold-tolerant and high-yield Volvariella volvacea strain. [Methods] In this study, the high-yield strain Pingyou No. 1 tested by cultivation experiment and the cold-tolerant strain VH3 were chosen as crossing parent strains. Then used the molecular marker of mating-type genes in Volvariella volvacea to verify mating-type of Volvariella volvacea monospore strain and completed the cross breeding. At last part of the cold-intolerant hybrids were eliminated before cultivation experiment using techniques of fast screening of the cold-tolerant Volvariella volvacea strain and identification of the hybrid strains. [Results] The result of cultivation experiment showed that the strain Pingyou No. 1 got the highest biological efficiency, which would be chosen as the high-yield parent strain. The 172 cold-tolerant hybrid strains were screened out from the original 496 possible hybrid strains so that the workload of cultivation experiment in the late would get a 65% reduction. The result of hybrid strains cultivation experiment showed that VV093 got higher biological efficiency than Pingyou No. 1 or VH3 at 28 °C. [Conclusion] Establishment of an efficient cross breeding method assisted by molecular markers of Volvariella volvacea included selection of parental strains, identification of monospore strains’ mating-type genes, monospore cross breeding, screening and identification of possible hybrid strains, and it was applied to monospore cross breeding for the cold-tolerant and high-yield Volvariella volvacea strain successfully.