Screening and identification of an ochratoxin A-producing Aspergillus niger strain

Abstract:

[Objective] The purpose of this study is to screen the fungus strain that can produce ochratoxin A (OTA). [Methods] To this end, 32 fungal samples in our laboratory were cultivated in CYA and YES media, and screened using the high performance liquid chromatography with fluorescence detection, then rescreened using high performance liquid chromatography-mass spectrometry; According to the colony morphology, mycelial and conidia morphology as well as nucleotide sequence analysis of ITS, β-Tubulin gene and Calmodulin gene, the target strain was identified. [Results] One strain which could grow well in CYA, YES and CA media at 25 °C was selected and named as strain 1062. Strain 1062 was identified as Aspergillus niger species by cultural and morphological characteristics and phylogenetic analysis based on ITS rDNA, β-tubulin and Calmodulin gene sequences. [Conclusion] The strain 1062 is A. niger which can produce OTA.