Isolation and screening of bagasse-cellulose degrading-bacteria from seawater environment

Abstract:

[Objective] The aim of this study was to screen bagasse-cellulose degrading-bacteria from seawater environment and to research the influence on bagasse cellulose enzymatic activity by mixed-fermentation of strains. [Methods] Cellulose congo-red culture medium was used to screening, and extracellular enzyme activity as re-screening methods. [Results] Two strains (Z4 and S5) were isolated and identified as Bacillus licheniformis by 16S rRNA gene sequence similarity analysis. Strain S5 possessed the highest filter paper activity (FPA activity) and bagasse cellulose activity which were 1.16 U/mL and 2.80 U/mL respectively. Mixed-fermentation of Z4 and S5 could obviously increase in cellulose enzyme activity. FPA activity and bagasse cellulose activity increased by 40.60% and 14.21% compared to S5. Mixed-fermentation of S5 and Bacillus BZ5 increased FPA activity and bagasse cellulose activity by 6.23% and 25.92% respectively. [Conclusion] Mixed-fermentation can significantly improve the ability of cellulose degradation, Z-4 and S-5 have a potential application in mariculture.