Effect of Trichoderma viride on resolving cell wall of Chlorella

Abstract:

[Objective] We studied the effect of Trichoderma viride on resolving Chlorella cell wall. [Methods] The cell wall of Chlorella was resolved by the secretion from free T. viride or immobilized T. viride in sodium alginate and calcium chloride. The activity of cellulases secreted by free T. viride or immobilized T. viride was determined by spectrophotometry. [Results] Cell wall of Chlorella was resolved by 86.5% by the secretion of free T. viride, 34.3% higher than that by cellulases R-10 containing only β-glucosidase. Compared to cellulases R-10, T. viride secretion contained three cellulases, namely, endo-glucanase, exo-glucanase and β-glucosidase. This was probably the reason why T. viride secretion had higher rate of resolving cell wall. Immobilized T. viride could be used repeatedly at least 5 times. The rate of resolving Chlorella cell wall by immobilized T. viride in the first time and the fifth time was 81.5% and 52.1% respectively. [Conclusion] Cell wall of Chlorella can be resolved by the secretion both from free T. viride and immobilized T. viride.