Whole-genome sequencing and analysis of an endophytic actinomycete Streptomyces sp. PRh5 from Dongxiang wild rice

Abstract:

[Objective] Streptomyces sp. PRh5 is an endophytic actinomycete from Dongxiang wild rice, and shows strong antimicrobial activity in vitro. In order to further study Streptomyces sp. PRh5 antimicrobial mechanism and secondary metabolites biosynthetic gene clusters, it is necessary to decipher the strain genome. [Methods] The genome was sequenced using high-throughput sequencing, and then analyzed using relevant software for genome assembly, gene prediction and functional annotation, cluster of orthologous group (COG) cluster analysis, synteny analysis and total secondary metabolite biosynthesis gene clusters prediction. [Results] The whole genome was assembled into 290 contigs, and the genome size is about 11.1 Mb with GC content of 71.1%. This Whole Genome Shotgun project has been deposited at GenBank under the accession JABQ00000000. According to genome sequences analysis, 50 secondary metabolite biosynthetic gene clusters were predicted. [Conclusion] The results will provide genome sequences for future functional genomics, biosynthetic pathways and heterologous expression of secondary metabolites on Streptomyces sp. PRh5.