A systematic review of the research progress and improvement of loop-mediated isothermal amplification

Abstract:

The loop-mediated isothermal amplification (LAMP) technique has been widely applied in the detection of nucleic acid because of its high specificity and sensitivity, time efficiency, and independent of thermo cycling equipment. After the invention of LAMP many studies have been done to modify and improve this method. This article has summarized these studies and reviewed the recent developments of LAMP technology from the following four aspects: methodology improvements; Shortening of reaction time and simplification of templates preprocessing; multiple products analysis; inhibition of false positive pollution. The objective of this review is to provide the theoretical foundation for the development of future novel nucleic acid detection platforms which will based on the LAMP technique.