Isolation and identification of pathogens from rotted root of Pinellia ternata in Guizhou province

Abstract:

[Objective] To identify microbes from rotted root of Pinellia ternata, so as to provide clues for efficient prevention of this plant disease. [Methods] Pathogens were isolated and their pathogenicity was confirmed by Koch’s rule. These pathogens were further studied through etiological, morphological and bacteriological techniques. In addition, 16S rRNA sequence analysis was done for bacterial pathogens, ITS and TEF sequence were used analysis for fungal pathogens. [Results] Twelve strains of bacterial pathogens and five strains of fungal pathogens were found in rotted tuber of P. ternata from three different cultivating places in Guizhou. The twelve strains of bacterial pathogens belonged to Pectobacterium carotovorum subsp. carotovorum. Among the fungal pathogens, ZJ and Z3 belonged to Fusarium oxysporum, and D3, D5 and H1 belonged to F. solani. [Conclusion] The pathogens that cause root-rot of Pinellia ternata in Guizhou include bacterial pathogens and fungal pathogens.