Detection and separation of the siderophores from Synechococcus sp. PCC 7002

Abstract:

[Objective] In order to provide a scientific basis for further research of marine siderophores, detection and separation methods of the siderophores from Synechococcus sp. PCC 7002 were investigated. [Methods] Synechococcus sp. PCC 7002 was cultivated in iron-limited Medium A. We used two-layer plate, mixture plate and traditional chromazurol S plate to detect the siderophores qualitatively. The relative amount of siderophores was detected by the chromazurol S shuttle assay. Amberlite XAD-2 resin and immobilized metal-chelating affinity chromatography were used for siderophores separation. Two different elution methods (pH reduced elution and competitive elution) were utilized in immobilized metal-chelating affinity chromatography. [Results] Mixture plate was more rapid, efficient and convenient among the three qualitative detection methods. The relative amount of siderophores from the iron-limited culture was up to 93.50%. Siderophores adsorbed sufficiently into Amberlite XAD-2 resin and the separating efficiency was relatively high when the sample pH was adjusted to 2. Strong fluorescence of siderophores from Synechococcus sp. PCC 7002 was observed at 254 nanometer ultraviolet light. [Conclusion] In present study, we got the effective detection and separation methods of siderophores from Synechococcus sp. PCC 7002.