Indole antibiotics from entomopathogenic bacterium Xenorhabdus bovienii PacYellow

Abstract:

[Objective] To study secondary metabolites of special eco-environmental microorganism— Xenorhabdus bovienii PacYellow, and to provide structurally diverse compounds for microbial drug/pesticide development. [Methods] The strain PacYellow was identified by comparison of 16S rRna gene. The secondary metabolites were purified by thin-layer chromatography, silica gel column chromatography, gel chromatography, and semi-preparative high-performance liquid chromatography. The structures were elucidated by using spectroscopic technologies including nuclear magnetic resonance (NMR), infrared spectroscopy (IR) and mass spectroscopy (MS). [Results] The strain PacYellow was identified as a strain of Xenorhabdus bovienii. Four compounds were purified and their chemical structures were elucidated as indole derivatives that are probably biosynthesized from tryptophan and valine or isoleucine. [Conclusion] Four indole derivatives were identified from entomopathogenic bacterium Xenorhabdus bovienii PacYellow.

Key words:Xenorhabdus spp., Natural products, Indole alkaloids, Antibiotics