Distribution characteristics of intracellular and extracellular lead of strain (Fusarium sp.) determined by EDTA method

Abstract:

[Objective] To establish a kind of technology which is able to measure the distribution of the intracellular and extracellular Pb2+ of Fusarium sp.. [Methods] Soaked the mycelium pellet in EDTA solution to elute and measure the extracellular Pb2+, then digested the soaked mycelium pellet to measure the intracellular Pb2+. [Results] EDTA can elute the extracellular Pb2+, but the cells will not be damaged in 99 minutes; and that it is feasible to measure the concentrations of Pb2+ ions within cells, on the surface of cells and in the medium, with EDTA as reaction medium and titrant and XO as the indicator. On the basis of this experimental method, the growth curve of the strain in medium with 500 mg/L Pb2+ was determined, and the Pb2+ concentrations of medium, and intracellular and extracellular lead contents were measured. [Conclusion] Pb2+ will be adsorbed on the surface of cells at first, and then transported into cells. Results show that the Pb2+ adsorbance on the surface of fusaria cells is 1.37 mg/g, and the Pb2+ adsorption site on the surface of fusaria cells are about 3.97×1018/g.