Effect of antibiotics or high-fat diet on diversity of intestinal Lactobacillus spp. in rat

Abstract:

[Objective] This study aimed to investigate the diversity of intestinal Lactobacillus in normal diet rats (NDR), high-fat diet rats (HFDR) and antibiotic diet rats (ADR). [Methods] Culture-dependent and culture-independent methods (16S rRNA gene sequencing, DGGE and q-PCR) were applied to isolation, identification and diversity analysis of intestinal Lactobacillus in above three groups. [Results] According to the 16S rRNA gene sequencing analysis, L. johnsonii, L. murinus, L. acidophilus, L. reuteri, L. plantarum, L. intestinals, L. animalis and L. vaginalis were isolated from the intestinal of NDR. Whereas L. animalis were not able to find in HFDR, L. intestinals and L. vaginalis were not isolated from ADR. The DGGE results showed that there was an obvious contrast in the composition of intestinal Lactobacillus among 3 groups, and a high similarity in the same group as well. Comparing the richness of Lactobacillus in three groups, ADR was the lowest one. Moreover, the intestinal Lactobacillus of NDR owed the highest diversity than the others. The q-PCR data indicated that the population of Lactobacillus in NDR was higher than that in other two groups, there was a significantly different (P<0.01) among three groups. [Conclusion] The diversity of the intestinal Lactobacillus can be reduced with the interference of high-fat diet or antibiotic.