Bacterial diversity in Bole river entrance soil of Ebinur Lake wetland, Xinjiang by 16S rRNA gene sequence analysis

Abstract:

[Objective] In order to investigate bacteria diversity and community composition in Bole river entrance soil of the Ebinur Lake wetland National Nature Reserve, Xinjiang. [Methods] Total DNA was directly extracted from the soil of the Ebinur Lake wetland Bole river entrance using the culture independent method. 16S rRNA gene was amplified using bacterial primer set Eubac27F and Eubac1492R. 16S rRNA gene clone library was constructed. Positive clones were identified by amplified rDNA restriction analysis (ARDRA) using Msp I and Afa I, and unique rDNA pattern clones were sequenced, analysed, and then the phylogenetic tree was constructed. [Results] seventy-five different clones of Macrorestriction Map was classified into 58 operational taxonomic units (OTU), which were associated with 8 phyla by phylogenetic analysis, including Chloroflexi, Cyanobacteria, Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Verrucomicrob and Gemmatimonadetes. Among them, the most abundant phyla was Proteobacteria, followed by Bacteroidetes, accounting for about 65% of the total clones. [Conclusion] The bacterial diversity is abundant in the Ebinur Lake wetland Bole river entrance soil, and exist a certain amount of new unknown taxon in this environment.