Isolation and identification of a seaweed polysaccharide degrading bacteria strain

Abstract:

[Objective] A bacterial strain, L206, which could degrade seaweed polysaccharides was isolated from the rotten brown algae. This research aims to analyze its ability to degrade different polysaccharides of seaweed. [Methods] The morphologic, biochemical and physiological characteristics and 16S rRNA gene were analyzed to identify the taxonomic position of strain L206. Then the activity of seaweed polysaccharide degrading enzyme was measured by DNS. [Results] The bacterial strain was a Gram-negative short bacillus. Its logarithmic growth phase was 3?21 h, with NaCl concentration range from 0 to 3% (w/v) for suitable growth. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that the strain L206 was Agarivorans albus. The comprehensive enzyme activity reached maximum level after strain L206 was induced for 72 h by the powder of Laminaria japonica, amylase present the highest enzymatic activity (28.17 U/mL), and followed by xylanase (23.83 U/mL). [Conclusion] As a multi-functional polysaccharide-degrading bacterium, Agarivorans albus L206 shows a special ability to degrading brown algae polysaccharide and has a great development potential.

Key words:Marine bacteria, 16S rRNA gene, Polysaccharide-degrading enzyme, Enzyme activity