Polymorphism analysis of genomic SSR markers of Volvariella volvacea by electronic PCR

Abstract:

[Objective] To verify the polymorphism of SSR markers analyzed with electronic PCR in Volvariella volavacea genome by using real PCR. [Methods] SSR loci in V. volvacea genome were identified with MISA software and the primers of the SSR molecular markers were developed with Primer3.0 software. The polymorphism of SSR markers were analyzed with electronic PCR and then verified by PCR. [Results] The 658 pairs of randomly selected SSR primers were checked by real PCR in 2 homokaryon strains, V23-1 and PD19, and the results show that 28.6% of SSR primers have the polymorphism. When the tested SSR loci belong to the type that had no length difference analyzed by the electronic PCR, only 4.8% SSR primers had the polymorphism. When the tested SSR loci selected from the group in which the length difference among the electronic PCR products of different genome was more than or equal to 3 bp, the true polymorphous SSR primers reached 48.3%. [Conclusion] The real PCR confirmed that it is more efficient to select polymorphous SSR markers with electronic PCR.

Key words:Volvariella volvacea, SSR, polymorphism, electronic PCR