Nutritional conditions for laccase production by Trametes hirsuta D2 in solid-state fermentation using hickory hull

Abstract:

[Objective] In order to increase the laccase productivity and reduce the production cost, solid-state fermentation (SSF) (using hickory hull) medium for Trametes hirsuta D2 has been studied. [Methods] The effects of supplementation with carbon and nitrogen sources, ratio of carbon and nitrogen source and the content of hickory hull on laccase production were investigated. [Results] The hickory hull was a good support for mycelial growth during laccase fermentation, and the corn flour and rapeseed meal were found efficient and selected as additional nutrition for laccase fermentation. The optimum solid materials in medium were consisted of 40% (w/w) hickory hull, 24% (w/w) corn flour and 36% (w/w) rapeseed meal. Under these conditions, the highest laccase activity (126.8 U/g dry substrate) was achieved after 6 days of cultivation. [Conclusion] The higher laccase productivity and lower production cost could facilitate industrial application of laccase.