Use of response surface methodology on the production of active compound MFS from Fusarium solani and its activity on HMG-CoA reductase

Abstract:

[Objective] In the present study, response surface methodology (RSM) was used to optimize the fermentation conditions of MFS (metabolite of Fusarium solani FG319) extracted from marine microorganism, Fusarium solani. [Methods] On the basis of single factor tests, Box-Behnken RSM was employed with three factors such as addition of L-ornithine hydrochloride at different concentrations, culture time, and culture temperature on the yield of MFS. [Results] The optimum culture conditions were as follows: the L-ornithine hydrochloride addition was 0.6% (W/V), culture time was 7 d, culture temperature was 22 °C. Under these conditions the yield of MFS was 20.11 mg/L, which is 4 times increased than without any optimization and the relative error with the theoretical value was 0.64%. The inhibition rate of MFS on HMG-CoA reductase was achieved above 100 mg/L, and has a similar inhibition as Lovastatin. [Conclusion] The culture conditions obtained by response surface methodology were effective and reliable, and the effect of MFS on HMG-CoA reductase is obvious in vitro.

Key words:Response surface methodology, Culture conditions, Marine microorganisms, HMG-CoA reductase