Analysis of microbial community structure in pit mud from two Chinese Luzhou-flavor liquor producing areas

Abstract:

[Objective] To explore the microbial community structures and diversity in pit mud from two typical Chinese Luzhou-flavor liquor producing areas, and to investigate the regional characteristic of microbial community and the effects on the formation of the liquor style. [Methods] Total DNAs were extracted from pit mud from Sichuan and Anhui liquor producing areas, respectively. Bacteria and Archaea were analyzed by PCR-ARDRA and 16S rRNA gene clone sequencing. [Results] The bacteria in pit mud from two Luzhou-flavor liquor producing areas were rich, including Firmicutes, Bacteroidetes, Chloroflexi, Synergistetes, Armatimonadetes and Unclassified bacteria. The predominant bacteria from both areas were clostridia in Firmicute. More Synthrophomonas and Petrimonas were detected in pit mud from Sichuan liquor producing area. Archaea composition was relatively simple. Methanoculleus, Methanosarcina, Methanosaeta and Methanobacterium were the major methane-producing archaeal group. The predominant archaea in Sichuan liquor producing area were Methanoculleus and Methanosarcina; while the predominant archaea in Anhui liquor producing area were Methanosarcina and Methanosaeta. [Conclusion] The 16S rRNA gene clone libraries not only systematically reflect the similarities and differences of microbial community between Sichuan and Anhui liquor producing areas, but also is valuable for revealing the formation of differences in liquor body style between two Chinese Luzhou-flavor producing areas.