Isolation and identification of bacterium strain efficient degrading formaldehyde and its degraing characteristics

Abstract:

[Objective] The purpose of this project was to isolate a newly formaldehyde-degrading bacterium from soil of dyeing and printing plant activated sludges. [Methods] Strain was isolated by pure culture method and classified based on standard morphological identification, physiological and biochemical tests, 16S rRNA gene sequence analysis and formaldehyde dehydrogenase genes amplification. And the orthogonal design was used to study the capability of degradation characteristics of strains W1. [Results] The strain was identified as Pseudomonas putida. The optimum conditions of formaldehyde degradation were as follows: concentration of formaldehyde 500 mg/L, incubation temperature of 30 °C, pH 6.0, rotating rate of 200 r/min, bottled fluid volume of 3%. [Conclusion] The results showed that under optimum conditions, strain W1 had strong formaldehyde degradation capability in this study. The removal efficiency of formaldehyde can be as high as 98% in 24 h.