Research advancement of molecular biological technology in the study of intestinal flora

Abstract:

Abundant of microorganism colonized in the gastrointestinal tract. They constructed a mutually dependent and restrained micro-ecological environment with the host. The composition of gut microbiota was closely related with the gene, dietary, health state and environment of the host. Furthermore, the gut microbiota was regarded as a post natal acquired organ and they played important roles in nutrient absorption and digestion, energy supply, fat regulating, intestinal epithelium renewal stimulating and immune system directing. This review focused on the research advancement of some modern molecular biotechnology in intestine microorganism research, including fluorescence in situ hybridization (FISH), polymerasechain reaction denaturing gradient gel electrophoresis (PCR-DGGE), real-time polymerase chain reaction (RT-PCR), gene chip and pyrosequencing platform as the representative of the second generation sequencing technology. In additions, it also made a future prospect for the study of intestinal flora.