A strain of marine low-temperature glucose oxidase screening, identification and the preliminary research of part enzymology properties

Abstract:

[Objective] To isolate strains producing glucose oxidase from the sample of sea mud and sea sand in Yellow Sea. [Methods] The strain isolated by plate culture and enyme activity determination, which was identified based on its morphology characteristics, physiobiochemical characteristics and 16S rRNA gene sequence analysis, parts of the glucose oxidase properties were studied, also. [Results] The strain GOD2 (glucose oxidase) was identified as Pseudomonas (Pseudomonas migulae), and the optimum reaction temperature of the enzyme was 20 °C, with the poor thermal stability, the relative activity of the remaining 80% at 40 °C. Enzyme activity decline rapidly above 40 °C. [Conclusion] GOD2 had a highly research value, and there is no report about it to produce glucose oxidase, at present.