Rapid epifluorescence enumeration of marine viruses: method optimization and application

doi:10.13344/j.microbiol.china.130297

Home > Archive>Volume 41, Issue 4, 2014 >776-785. DOI:10.13344/j.microbiol.china.130297

Rapid epifluorescence enumeration of marine viruses: method optimization and application
DOI:
                        10.13344/j.microbiol.china.130297
                    
CSTR:
                        32113.14.j.MC.130297
                    
Author:
                        XIAO Jin-ZhouXIAO Jin-Zhou
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Production on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
SUN Guo-WeiSUN Guo-Wei
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Production on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
WANG Hong-MingWANG Hong-Ming
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Production on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
ZHOU Jing-LieZHOU Jing-Lie
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Production on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China 4. College of Science and Mathematics, Auburn Univeristy, Auburn 36849, USA
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LI Bai-LinLI Bai-Lin
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
PAN Ying-JiePAN Ying-Jie
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Production on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
WANG Yong-JieWANG Yong-Jie
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Production on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site

                    
Affiliation:
Clc Number:
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

[Objective] To establish the epifluorescence microscopic (EFM) method for rapid and reliable enumeration of marine viruses. [Methods] Fixed sea water samples were filtered through 0.02 μm filter, and virus particles were collected on the membrane. After stained with SYBR Green I, viruses were observed and counted under epifluorescence microscope using a grid micrometer. [Results] The EFM method was optimized to count marine viruses rapidly and reliably. [Conclusion] The epifluorence enumeration method was established as well as applied to count marine viruses.

Key words:Marine virus, Enumeration, SYBR Green I

Get Citation

XIAO Jin-Zhou, SUN Guo-Wei, WANG Hong-Ming, ZHOU Jing-Lie, LI Bai-Lin, PAN Ying-Jie, WANG Yong-Jie. Rapid epifluorescence enumeration of marine viruses: method optimization and application[J]. Microbiology China, 2014, 41(4): 776-785

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:
Revised:
Adopted:
Online: April 04,2014
Published:

Get Citation

Share

Article Metrics

History

Article QR Code