Rapid epifluorescence enumeration of marine viruses: method optimization and application

Abstract:

[Objective] To establish the epifluorescence microscopic (EFM) method for rapid and reliable enumeration of marine viruses. [Methods] Fixed sea water samples were filtered through 0.02 μm filter, and virus particles were collected on the membrane. After stained with SYBR Green I, viruses were observed and counted under epifluorescence microscope using a grid micrometer. [Results] The EFM method was optimized to count marine viruses rapidly and reliably. [Conclusion] The epifluorence enumeration method was established as well as applied to count marine viruses.