Isolation and identification of a tylosin-degrading strain and its degradation characteristics

Abstract:

[Objective] This study was to isolate a tylosin-degrading strain from the soil deposited by tylosin pharmaceutical waste and investigated its degradation characteristics. [Methods] The strain was identified based on the morphology observation, experimental results of physiology and biochemsitry, and then the identification and phylogenetic analysis of the isolated strain was performed by 16S rRNA gene sequence, also, the characteristics of the strain in degradation of tylosin was investigated. [Results] A strian named TS1 was newly isolated from the soil deposited by tylosin pharmaceutical waste. The cells of the isolate were Gram-negative rods and the colony morphology was round, oyster white, opaque, and smooth with regular edge. The isolate was identified as Burkholderia vietnamiensis. This strain was capable of degrading 99% of tylosin in medium with an initial concentration of 300 mg/L after 72 h of incubation under conditions of the initial pH 7.0 and 35 °C. [Conclusion] The isolated strain has a high ability to degrade tylosin, and may be used for bioremediation of environment contaminated by solid or liquid waste containing tylosin residue.

Key words:Tylosin-degrading strain, 16S rRNA, Phylogenetic analysis, Bioderadation characteristics