Screening of fungal strains with laccase and optimization and identification of the strain with high-yield laccase

Abstract:

[Objective] To obtain the fungal stain with high-yield laccase. [Methods] Strains producing laccase were screened out by the guaiacol method. Then the strain with the high-yield laccase screened was optimized by the orthogonal experiment design and identified using the morphological characters and the molecular systematics. [Results] Four strains could produce laccase in these 26 fungal strains, and the strain H52.1 was the best high-yield strain, and its optimal medium components and conditions were as follows, the optimal carbon source was soluble starch, nitrogen source was ammonium nitrate, pH value was 8 and metal ion was Ca2+. The result of identification showed that the strain H52.1 was Taifanglania major. [Conclusion] Taifanglania major has been worth doing further research and exploitation in the laccase development.

Key words:Laccase, Optimization, Taifanglania, Taifanglania major