Lipids analysis of Isochrysis zhangjiangensis during cultivation process by HPTLC

Abstract:

[Objective] To study the lipid compositions changes of Isochrysis zhangjiangensis from nitrogen-sufficient to nitrogen-limited cultivation process. [Methods] High performance thin layer chromatography (HPTLC) was used to analysis lipids of microalga Isochrysis zhanjiangensis. [Results] With the consumption of nutrition in the medium, the cells were gradually subjected to stress conditions, under which condition a large quantity of triacylglycerol (TAG) was accumulated. Opposed the storage of TAG, the lipids composed the biomembrane, monogalactosyldiacylglycerol (MGDG), sulfoquinovosyldiacylglycerol (SQDG), phosphadidylglycerol (PG) and phosphatidylcholine (PC) gradually reduced. The content of free fatty acid (FFA), diacylglycerol (DAG), digalactosyldiacylglycerol (DGDG), phosphatilinosotol (PI) and phosphatidylethanolamine (PE) kept relatively stable. [Conclusion] As a robust and rapid tool for separation and analysis of lipids, HPTLC can be used in the study of lipid metabolism and regulatory accumulation of TAG in microalgae.

Key words:High performance thin layer chromatography (HPTLC), Lipid metabolism, Nitrogen limitation, Isochrysis zhangjiangensis