Antagonism on microorganisms and cancer cells by the fermentation broth of endophytic fungi from Tribulus terrestris L.

Abstract:

[Objective] The aim of the present study is to investigate the anti-microorganisms and anticancer activities of endophytic fungi isolated from Tribulus terrestris L.. [Methods] Endophytic fungi were cultured in PDB or Czapek medium to obtain different liquid fermentation. The activities of fermentative products were tested by using Oxford plate assay system, Magnaporthe oryzae P-2b model and cancer cell model. [Results] The strains with their inhibition zone diameter of the PDB and Czapek broth greater than 10 mm were accounted for 19.05% and 23.81% respectively of the total strains. The strains of which the PDB broth and the Czapek broth with the MIC (Minimum inhibitory concentration) of Magnaporthe oryzae lower than 10%, were accounted for 19.05% and 47.61% respectively of the total strains. The PDB fermentation broth with the inhibitory action on cancer cells over 50% was accounted for 52.38% of the total PDB broth, and the Czapek fermentation broth with the inhibitory action on cancer cells over 50% was accounted for 28.57% of the total Czapek broth. [Conclusion] A certain amount of fermentative broth of endophytic fungi isolated from Tribulus terrestris L. had effective anti-microorganisms and anticancer activities.