Analysis of energetic metabolism of Acetobacter pasteurianus during high acidic vinegar fermentation

Abstract:

[Objective] We are aiming to analyze the energy metabolism of A. pasteurianus CICIM B7003-02 during high acidic vinegar fermentation so that we can identify the targets to enhance the energy metabolism for vinegar productivity improvement. [Methods] Tricarboxylic acid cycle metabolism, alcohol respiratory chain enzyme activity and relative transcriptional level of enzyme-coding genes in energy metabolism pathways were investigated during the high acidic vinegar fermentation by B7003-02. [Results] We have characterized the bacterial energy metabolism during high acidic vinegar fermentation. Malic/succinic acid feedback pathway is the major route for energy supply at the beginning of fermentation, and alcohol respiratory chain plays the major role in providing energy to cells during the major acetification phase. Both malic/succinic acid feedback pathway and the alcohol respiratory chain supply energy when fermentation approaches its final stage. [Conclusion] Energy supply influences vinegar productivity, the supplementation of malic acid and succinic acid can strength the malic/succinic acid feedback pathway and improve vinegar productivity.