Eukaryotic microbes diversity in a disused thermal vent in Yangbajing, Tibet A. R.

Abstract:

[Objective] To discover the eukaryotic microbes diversity in sediment from a disused thermal vent in Yangbajing, Tibet A. R.. [Methods] To constructed the ITS-rRNA gene library of total DNA extracted from sediment by analyzed ITS-rRNA gene. Positive clones from the library were analyzed randomly by amplified ribosomal DNA restriction analysis (ARDRA). Phylogenetic trees based on eukaryotic ITS-rRNA gene sequences were built up by using MEGA 4.0 program. [Results] There were Ascomycota, Chytridiomycota, Basidiomycota, Cercozoan, Metazoan and uncultured fungus in sediment from Yangbajing thermal vent. Chytridiales sp., Physoderma maydis, Powellomyces sp., Candida parapsilosis, Zygosaccharomyces rouxii, Aureobasidium pullulans and Lecane bulla, all of them have not yet been reported to be inhabited in hot springs or thermal vents. [Conclusion] There were lots of eukaryotic microbes communities in sediment from the disused thermal vent in Yangbajing, Tibet A. R..

Key words:Thermal vent, ITS-rRNA gene, Eukaryotic microbes, Phylogenetic analysis