Proteomic analysis of cell wall proteins from Saccharomyces cerevisiae with different generation numbers

Abstract:

[Objective] To find out the mechanisms of yeast aging. [Methods] We performed continuous inoculation of Saccharomyces cerevisiae FFC2146 from generation to generation. We also observed the morphogenesis of Saccharomyces cerevisiae which has different generation numbers (2, 10, 15) through microscope, and measured absorbance in 600 nm to signify flocculation after sedimentation and determined residual glucose by DNS. Proteomic analysis of yeast cell wall proteins from the 2th and 15th generation was observed by Two-dimensional electrophoresis. [Results] Results showed that, the 15th generation yeast has rougher cell surface, higher flocculating velocity and lower metabolic level than the 2th and 10th generation, indicated that continuous inoculation accelerates yeast recession. Proteins were obtained by dithiothreitol and phenol abstraction. 309 proteins were detected and presented 11 changes responded to cell recession, 6 spots reduced by more than 2-fold in the 15th gel, 4 spots appeared in the 15th gel only and 1 spot appeared in the 2th only. [Conclusion] 11 Proteins from Saccharomyces cerevisiae cell wall changed after continuous inoculation 14 times, the changes of expression levels among these proteins were connected to yeast aging.

Key words:Saccharomyces cerevisiae, Yeast aging, Generation times, Cell wall proteins, Two-dimensional electrophoresis