Isolation, identification and the inhibition effect endophytic bacteria in Focus hispida fruit

Abstract:

[Objective] To provide a certain basis, for the relationship between the ecology of endophytic bacteria and plant as well as the resources of endophytic bacteria. [Methods] Use tradational cultural methods isolated 54 endophytic bacteria from the fruit of Focus hispida through the restriction enzyme analysis (ARDRA) told have 16 operational taxonomic units, 16s rDNA sequence system development analysis on 16 representative strains. [Results] The results showed that 16 strains were found the highest similarity of strains from GenBank, the identities are from 95% to 100%. six endophytic bacreia were classed to Bacillus genera, the identities are from 95% to 98%, was the dominant genus among the endophytic bacteria in Focus hispida fruit. Three were classed to Staphylococcus genera, one were classed to Pseudomonas genus, one was similar to uncultured bacterium, one were classed to Kocuria genus, one were classed to Delftia genus. [Conclusion] The phylogenic tree showed that the 16 endophytic bacteria clustered two main branches. the inhibition experiment of 14 endophytes bacteria, 13 strains have different degrees of inhibition effect. Especially the swx15 of Bacillus genus and the swx25 of Acinetobacter genus had the biggest inhibition spectrum.