Pseudomonas aeruginosa gene pfm affects the expression levels of type Ⅲ secretion system effectors

Abstract:

[Objective] Detect the effect of gene pfm on type Ⅲ secretion system effectors. [Methods] Complementary strain pfmC was constructed. Total RNA was extracted from the wild type strain PAO1, mutant strain Δpfm and complementary strain pfmC respectively and real-time PCR was performed to detect the transcription levels of exoS, exoT and exoY. Further more, both total intracellular proteins and secreted proteins of strains PAO1, Δpfm and pfmC were collected, and detected against the representative effector ExoS by western blot. [Results] The results showed that transcription levels of exoS, exoT and exoY were significantly decreased in Δpfm compared to PAO1, and recovered in pfmC. Western blot showed that both total intracellular and secreted ExoS of Δpfm were significantly lower than that of PAO1 and recovered in pfmC. [Conclusion] In conclusion, gene pfm of Pseudomonas aeruginosa can affect type Ⅲ secretion system effectors.