Optimization of culture conditions of the biocontrol strain CNY-04 against Botryis cinerea by response surface methodology

Abstract:

[Objective] In order to raising the antagonistic effect, the growth conditions of biocontrol strain CNY-04 was optimized. [Methods] The growth conditions of the biocontrol strain CNY-04 were optimized by response surface methodology basing on single factor tests. The growth curve was also measured. [Results] The optimal culture conditions of the biocontrol strain CNY-04 were determined for 0.5% glucose, 2.0% peptone, 0.1% yeast extract, 0.5% beef extract, temperature at 32 °C, pH 8.0, speeding at 150 r/min, the incubation time of 48 h, inoculation amount of 4%, liquid volume of 75 mL/250 mL. The OD600 of CNY-04 was 2.907 with the optimal growth conditions, consistenting with the predicted value. The antibacterial circle diameter was 44.5 mm, improving by 30.9%. [Conclusion] The optimal growth condition of the biocontrol strain CNY-04 was identified, providing a theoretical basis for enlargement of production.

Key words:Biocontrol strain, Response surface methodology, Botryis cinerea, growth curve