Isolation and identification of a fibrinolytic bacterium

Abstract:

[Objective] We isolated and identified strains producing fibrinolytic enzyme. [Methods] A strain with fibrinolytic activity was isolated from the environment by using blood powder plates and agar-fibrin plates. We identified the strain by morphological, physiological and biochemical characterization, combined with 16S rRNA gene sequence and phylogenetic tree. [Results] A strain (named EF608) with fibrinolytic activity was obtained and identified as Enterococcus faecalis. Fermentation broth of strain EF608 had fibrinolytic activity. Its optimal reactive temperature and pH value were 35 °C and 7.5, respectively, and the molecular weight is 37 kD by SDS-PAGE and fibrin autography. EDTA completely inhibited the fibrinolytic activity, but PMSF exerted little effect on it. The fermentation broth dissolved thrombus in vitro, directly degrade the fibrin, and did not hydrolyze blood cells. [Conclusion] The strain might provide a new source for novel fibrinolytic enzymes.