Optimization of xylanase production from Malbranchea cinnamomea by solid state fermentation

Abstract:

[Objective] We optimized xylanase production from Malbranchea cinnamomea CAU521 by solid state fermentation (SSF) with agricultural wastes as carbon source. [Methods] single-factor experiment was used to optimize various factors affecting the enzyme production. There are six factors which include carbon source, nitrogen source, initial pH, initial moisture content, temperature and fermentation time were investigated. [Results] the optimum conditions for xylanase production were as follows: rice straw as carbon source, 2%?(w/w) yeast extract as nitrogen source, the ratio of solid substrate to mineral solution of 1:4 (W/W), the initial pH of 7.0, and incubation temperature of 45 °C. Under the optimized conditions, the highest xylanase production of 13 120 U/g dry carbon source was achieved after 6 days of cultivation. [Conclusion] the high-level xylanase production and low production cost may facilitate industrial scale enzyme application.