The selective isolation of endophytic actinomycetes

Abstract:

[Objective] In order to obtain more resources of endophytic actinomyctes, the aim of the study is to establish an effective method of isolating endophytic actinomycetes. [Methods] Disinfectant and sterilization procedures, pretreatment method, isolation medium were compared. The isolates were identified based on morphology and 16S rRNA gene sequence analysis. [Results] It is very effective for samples sterilized 4?7 minute with 5% NaClO. The pretreatment method of processing sample 15 minutes at 100 °C have a good effect on reducing the interference of fungi and bacteria. Isolation mediums containing sodium propionate and sodium succinate have good effect on isolation. [Conclusion] The isolation method that plant samples were surface sterilized and dried, processed 15 minutes at 100 °C, broken in a sterile mixing cup, and then were separated on isolation medium is very useful.