Resistance analysis of Salmonella Indiana to the chlmphenicols

Abstract:

[Objective] To investigate the characteristics of chloramphenicols resistance and the prevalence of resistance genes in Salmonella Indiana from chicken. [Methods] The samples were isolated from chicken hatcheries, farms and slaughterhouses in Shandong Province. The isolates were identificated and tested the drug susceptibility, designing primers for the resistance genes of chloramphenicols in strains were detected by PCR and sequence analysis. [Results] The results showed that the isolation rate of Salmonella Indiana was 23.28%. The resistance rates of Salmonella Indiana from hatcheries, farms, slaughterhouses to chloramphenicol were 50%, 83.33%, 93.30%, florfenicol were 83.33%, 100%, 100%, thiamphenicol were 63%, 65%, 77% respectively. In 80 chloramphenicols resistant strains, 54 strains had detected catA1 gene, 74 strains had detected floR gene, and the 5 strains had detected cmlA gene. [Conclusion] These results indicated that the resistance rates of Salmonella Indiana to chloramphenicols were different from hatcheries, farms and slaughterhouses. The catA1 gene and floR gene were widely existed in chloramphenicols resistant strains.