Identification of marine bacteria SSQ500 and the antitumor mechanism of fermentation products

Abstract:

[Objective] To identify the marine bacteria SSQ500 with antitumor activity, and to study the antitumor mechanism of fermentation products. [Methods] The strain SSQ500 was identified using its morphological characteristics, physiological, biochemical properties and 16S rDNA gene sequence. The tumor cell was collected after different concentration activity compounds acted on difference time. The inverse proportion of apoptosis of the liver cancer cell SMMC-7721 was analyzed by flow cytometry. To study the protein expression by examine Caspase3, Caspase8. [Results] The strain SSQ500 was identified belong to Myxococcaceae sp.. The activity compound SSQ produced by this bacteria can induce SMMC-7721 cell to apoptosis by up-regulate the expressions of Caspase3, Caspase8. [Conclusion] The SSQ500 belongs to Myxococcaceae sp., and the fermentation product of this bacteria can induce tumor cell to apoptosis. That may be related to up-regulate the expressions of Caspase3, Caspase8.