Isolation and diversity of crude oil-degrading bacteria from Dalian Bay

Abstract:

[Objective] In order to investigate the diversity of crude oil-degrading bacteria in the seawater, mud and sponge samples from Dalian Bay, and obtain new oil-degrading bacteria. [Methods] Crude oil was used as sole carbon source to enrich and isolate the potential oil-degrading bacteria from samples of seawater, mud or sponge. Phylogenetic analysis was conducted by 16S rRNA gene sequences of the bacteria. [Results] After screening via morphological and 16S rRNA gene sequences analysis 50 strains belonging to 22 genera were obtained. Among them, 6 strains shared 16S rRNA gene sequence identities of 95%?97% with the most similar strains, were speculated new species. Further experiments revealed oil-degrading capability of 45 strains. [Conclusion] This study demonstrated a high diversity of the cultivable oil-degrading bacteria in Dalian Bay, and obtained new resources in bioremediation of marine oil pollution.