Identification of a psychrotroph Pseudogymnoascus roseus HD1031 and the properties of its producing cellulase

Abstract:

[Objective] To screen a psychrotolerant cellulase-producing strain from the decayed soil of Huanglong Mountain, Sichuan, China, and determine the properties of the cellulase. [Methods] The strain was identified by the morphology and ITS sequence analysis. The DNS method was used to determine the activity of cellulases. [Results] A psychrotolerant cellulase-producing strain HD1031 was screened from the decayed soil of Huanglong Mountain. It was identified as Pseudogymnoascus roseus by the morphology and ITS sequence analysis. The strain could grow between 4 °C and 25 °C and its optimum growth temperature is 16 °C?17 °C. In the medium containing Avicel, corn cob powder, (NH4)2SO4 and Tryptone, the strain HD1031 produced cellulases. After cultivating under the conditions of 17 °C and 160 r/min for 8 days, the cellulases activities in the fermentation broth were CMCase activity 366.67 U/mL, FPA 87.6 U/mL and the β-glucosidase activity 90.8 U/mL. The optimum pH value and temperature of the CMCase was pH 6.0 and 50 °C, respectively. [Conclusion] A psychrotolerant cellulase-producing strain HD1031 was screened and identified. The cellulase possessed the characters of cold-active cellulase.

Key words:Pseudogymnoascus roseus, Psychrotroph, Cold-active cellulase