The optimization on laccase production from Trametes hirsuta and its property in the decolorization of synthetic dyes

Abstract:

[Objective] To improve laccase production and apply it to decolorize synthetic dyes, we made this research. [Methods] The nutritional and environmental conditions for laccase production were investigated. [Results] The optimum conditions described as follows: corn flour 20.0 g/L, potato starch 32.4 g/L, ammonium tartrate 2.9 g/L, Tween 80 0.5 g/L, CuSO4?5H2O 2.0 mmol/L, vanilline 0.54 mmol/L, NaH2PO4?2H2O 2.0 g/L, MgSO4?7H2O 0.5 g/L, MnSO4?H2O 0.1 g/L, inoculation concentration 8%, pH 6.0, liquid volume 40/250 mL, culture temperature 30 °C. [Conclusion] With the conditions, the activity of laccase was achieved at 35 U/mL, was higher 39 times than control. The laccase was applied to decolorize synthetic dyes and it was found that the laccase could decolorize azo dyes AR1 and RB5 rapidly which could be completed in 5 minutes.