b-Rhizobia associated with Mimosa spp. from Dehong district of Yunnan province analyzed by 16S rDNA PCR-RFLP and SDS-PAGE

Abstract:

[Objective] Genetic and phenotypic diversity of β-rhizobia associated with Mimasa spp. in Dehong district of Yunnan province were performed to revealed the diversity of rhizobial resources in China. [Methods] 60 isolates were analyzed by 16S rDNA PCR-RFLP, total cell protein SDS-PAGE analysis and 16S rDNA sequencing. [Results] All the isolates were clustered two genetic groups and two phena groups respectively, by characterizing 16S rDNA PCR-RFLP and SDS-PAGE anlaysis. The strains in each group are in coinciding with between two methods. All strains clustered with type strain of Cupriavidus, and Burkholderia. From the phylogenetic tree of 16S rDNA sequence, the test strains were belonged to the species of Cupriavidus taiwanensis, Burkholderia mimosarum and Burkholderia phymatum. [Conclusion] At present study, we find that β-rhizobium from Dehong district were belonged to Cupriavidus and Burkholderia mainly. The Cupriavidus spp. were predominated in these strains. These records showed the diversity of Rhizobium from Mimosa spp. and enriching resources of β-rhizobium in China.