Effect of co-expression of chaperones on Rhizopus oryzae recombinant lipase production in Pichia pastoris

Abstract:

[Objective] The aim of this study is to improve the yield of Rhizopus oryzae lipase in Pichia pastoris by co-expression of chaperones. [Methods] By high-density fermentation in 7 L bioreactor, the expression of lipase in strain BH128 co-expressed with two chaperones Ero1p and PDI was compared with that of H238 co-expressed without any chaperone. [Results] The results showed that the highest lipase activity, the specific growth rate, the specific production rate and the specific consumption rate in BH128 reached 2 388.7 U/mL, 0.02 h?1, 944.5 U/(gDCW·h) and 0.15 gmethanol/(gDCW·h), which is 1.7, 0.5, 4.1 and 1.3 fold higher than those in H238, respectively. Moreover, the fermentation period in BH128 was 20 hours shorter than in H238. [Conclusion] The high-level expression of Rhizopus oryzae lipase could be achieved by co-expression of chaperones Ero1p and PDI, which provided the potential application of Rhizopus oryzae lipase in industry.