Cu2+ immobilization of glucoamylase on metal chelating carries

Abstract:

[Objective] A chelate carrier with agarose containing Cu2+ iminodiacetic acid (IDA) for hydrolysis saccharification enzyme was prepared and the fixed the glucoamylase process conditions with the chelate carrier was optimized. [Methods] Based on the technology of protein separation with immobilized mental ion affinity chromatography, the method for immobilization of glucoamylase has been selected, and the enzyme activity of the immobilized glucoamylase was determined using the UV spectrophotometric for research the process influencing factors. [Results] The optimum immobilization conditions of enzyme were as flows: enzyme load of 80 mg/g carrier, Cu2+ of 1.0×10?2 mol/g carriers, immobilized time of 4 h, pH value of 4.6, and the activity of immoblized glucoamylase was 252.1 U/g. The activity of immoblized glucoamylase on the regenerated matrix keep 65.1% effective of the new enzyme activity after used 5 times. [Conclusion] The Cu2+-IDA-agarose magnetic metal chelate can be used excellent immobilization carriers for starch hydrolysis saccharification enzyme.