Rapid differentiation and identification of five species of Salmonella by FT-IR spectroscopy

Abstract:

[Objective] To establish a standard fourier transform infrared (FT-IR) spectral library and a FT-IR method of differentiation and identification of five species of Salmonella, viz Salmonella typhimurium, Salmonella enteritidis, Salmonella choleraesuis, Salmonella arizonae and Salmonella potsdam. [Methods] FT-IR fingerprint absorption spectra of five species of Salmonella were collected and analyzed by chemometric methods. [Results] A standard FT-IR spectral library was created and could be used to compare with those of the target Salmonella and identify them; two cluster models of principal component analysis (PCA) and hierarchical cluster analysis (HCA) were established and could differentiate and identify well the five species of Salmonella. [Conclusion] As a rapid, easy-to-use and accurate technique, FT-IR spectroscopy is an effective tool to differentiate and identify five species of Salmonella studied here.