Establishment of molecular ID in 11 Ganoderma lucidum strains

Abstract:

[Objective] Eleven Ganoderma lucidum strains were collected as materials for classifying them at the molecular level and establishing the molecular ID. [Methods] Internal transcribed spacer (ITS) and simple sequence repeat (SSR) markers were used for the molecular identification of eleven Ganoderma lucidum strains. [Results] 99% similarity in ITS sequence between the tested strains and the Ganoderma lucidum registered in GenBank, meaning that the tested strains were Ganoderma lucidum species. The cluster analysis by NTSYS revealed that eleven Ganoderma lucidum strains were divided into four groups at similarity coefficients of 0.62. GL-2 and GL-4 were in two clades respectively. According to fragment size of allele variation, the agarose gel electrophoresis bands were analyzed by the software ID Analysis 1.0. Five primer pairs could be used to identify all the tested strains and accomplish the establishment of molecular ID. [Conclusion] Establishment of molecular ID in Ganoderma lucidum based on SSR were feasibly.