Inhibitory of Bacillus vallismortis to Trichoderma parasitized in Lentinus edodes

Abstract:

[Objective] The inhibition of Bacillus vallismortis to Trichoderma was researched in this article, because Trichoderma was a harmful bacterial with a higher infection rate in the mushroom production and was normally killed by chemical agent with the result of pesticide residues. [Methods] Firstly, the components of Bacillus vallismortis were confirmed if they were the intracellular or extracellular materials. Then the stability of the physical and chemical properties of the effective component was analyzed after extraction. Finally, initial application in the mushroom cultivation was probed. [Results] The lipopeptide extracted from exocellular secretion of Bacillus vallismortis can effectively inhibit the germination of spores and mycelial growth. The inhibition rate could reached 62.8% with 0.02 g/L, little influence on the growth of mushroom mycelium, relatively stable physical and chemical properties. An addition of 26.6% 36 h fermentation broth of Bacillus vallismortis in the mushroom cultivation compound had the optimum inhibition on Trichoderma hyphae. [Conclusion] Extracellular secretions of Bacillus vallismortis had good inhibition on Trichoderma. It was worth further investigating considering the reduction of pesticide residues, though the mushroom mycelium germination was also inhibited at a certain degree.