Isolation, identification and biological characterization of Riemerella anatipestifer from goose

Abstract:

[Objective] To identify pathogenic bacteria isolated from dead goslings suspected Riemerella anatipestifer disease. [Methods] The isolated bacterial strain was subjected to examine by the cultural performances, biochemical properties, animal text, serological identification and biological features. [Results] The results showed that the isolated strain was identified as gram-negative bacteria, which could not ferment and alcohols, and it was positive of ureolysis test and oxidase test. Pathogenic. The multiple sequence alignment from different isolated stains 16S rRNA genes showed, the strain in this study was at the same evolution branches with the Riemerella anatipestifer isolated from ducks, but it was in little relation to the Riemerella anatipestifer isolated from chicken. It belongs to the serotype I of Riemerella anatipestifer. [Conclusion] The isolated bacterium belongs to the serotype I of Riemerella anatipestifer, which was highly pathogenic to the ducking and goosing, and the self-development vaccine could protect the goslings effectively against to be infected by Riemerella anatipestifer.