Screening and identification of plant growth-promoting rhizobacteria from Cerasus pseudocerasus rhizosphere

Abstract:

[Objective] The aim of the present study was to provide the excellent strain resources for the production of Cerasus pseudocerasus bio-fertilizer. [Methods] Bacterial isolates were isolated and screened from the rhizosphere soil of Cerasus pseudocerasus using the methods of remaining green and radish cotyledon bioassay. And then, plant hormones produced by the bacterial isolates were quantitatively detected. Additionally, a pot experiment was conducted to determine whether the use of the bacterial isolates benefits the growth of Cerasus pseudocerasus. At last, the bacterial isolates that exert beneficial effects were identified based on the results of morphologic characteristics, physiological biochemical properties and phylogenetic analysis of 16S rRNA genes. [Results] Four plant growth-promoting rhizobacteria (PGPR) with potential beneficial effects on Cerasus pseudocerasus growth were screened. According to the pot experiment results, AI5, AI21, PII17, and PI7, especially PII17 can significantly improve the root and aerial part biomass of Cerasus pseudocerasus, showing the beneficial effects on Cerasus pseudocerasus growth. AI5 and AI21 were identified as Bacillus pumilus. PII17 and PI7 were identified as Pseudomonas sp. and Enterobacter sp., respectively. [Conclusion] AI5, AI21, PII17, and PI7, especially PII17 that exert beneficial effects on Cerasus pseudocerasus growth exhibited broad application prospects in the bio-fertilizer production.