Molecular identification of jujube witches’-broom phytoplasmas strains from Tai’an

Abstract:

[Objective] To identify the three jujube witches’-broom phytoplasmas strains from Tai’an. [Methods] The phytoplasmas strains were identified by 16S rDNA sequence analysis via PCR amplification of 16S rDNA gene with the universal primer pair of R16F2n/R16R2 for phytoplasmas and online virtual 16S rDNA-RFLP analysis, and then their 16S rDNA sequences were deposited in GenBank. [Results] The results showed that the 16S rDNA sequences of the three strains shared the identity of 99.5%?99.7% with that of jujube witches’-broom phytoplasmas (AB052876 and AF279272), cherry lethal yellows phytoplasmas (AY197659) and apricot leaf roll phytoplasmas (FJ572660) which belong to 16Sr Ⅴ-B subgroup, and they were named as jujube witches’-broom phytoplasma strain Yuanling1 (JWB-Yuanling1, TA), jujube witches’-broom phytoplasma strain Lubeidongzao (JWB-Lubeidongzao, TA) and jujube witches’-broom phytoplasma strain Dabailing (JWB-Dabailing, TA) respectively. The 16S rDNA sequences of three JWB strains were deposited in GenBank with the accession numbers of HM989946, HM989947 and HM989948 respectively. [Conclusion] The three phytoplasmas strains from Tai’an were identified as the members of 16Sr Ⅴ-B subgroup.